|
Eighteen in-vitro and ten in-vivo assays have been standardized in our laboratory. Many of
these are routinely used for the evaluation of plant extracts, pure isolated constituents
and synthesized compounds to take forward researches in drug discovery and development
programs.
In-vitro Assays
| 1.
|
Cytokines assay (TNF-α)
|
Anti-inflammatory
|
| 2.
|
Cytokines assay (IL-1β)
|
Anti-inflammatory
|
| 3.
|
Cytokines (IL-2)
|
Anti-inflammatory
|
| 4.
|
Pancreatic Lipase inhibitory assay
|
Anti-obesity
|
| 5.
|
Adipolytic Assay
|
Anti-obesity
|
| 6.
|
DPPH assay
|
Anti-oxidant
|
| 7.
|
NBT reduction assay
|
Anti-oxidant
|
| 8.
|
FTC assay
|
Anti-oxidant
|
| 9.
|
TBA assay
|
Anti-oxidant
|
| 10.
|
Chemiluminence
|
Anti-oxidant
|
| 11.
|
NO assay
|
Anti-inflammatory
|
| 12.
|
Cell-viability assay (MTT)
|
Cytotoxicity
|
| 13.
|
BST assay
|
Toxicity
|
| 14.
|
Estrogen assay
|
Gynecological disorders
|
| 15.
|
α-Glucosidase inhibitory activity
|
Anti-diabetic
|
| 16.
|
α-Amylase inhibitory activity
|
Anti-diabetic
|
| 17.
|
Fructose-2,6-biphosphate production assay
|
Anti-diabetic
|
| 18.
|
Bioavailability
|
In human blood
|
In-vivo Assays
| 19.
|
Swimming Endurance assay
|
Anti-stress
|
| 20.
|
Corticosterone assay
|
Anti-stress
|
| 21.
|
Cell-mediated immunity
|
Immunity
|
| 22.
|
Humoral immunity
|
Immunity
|
| 23.
|
LH/FSH and prolactin assay
|
Gynecological disorders
|
| 24.
|
Estrogenic(ovarectomized)
|
Gynecological disorders
|
| 25.
|
Estrous cycle assay
|
Gynecological and anti-fertility
|
| 26.
|
Progesterone assay
|
Anti-fertility
|
| 27.
|
Streptozotocin induced diabetes
|
Anti-fertility
|
| 28.
|
Carrageenin induced paw odema
|
Anti-inflammatory
|
TOP
The following plant materials have been subjected to above mentioned putative biological/pharmacological activities and were studied in detail:
|
1. |
Achyranthes aspera
|
|
2. |
Acorus calamus
|
|
3. |
Aegle marmelos
|
|
4. |
Agaricus Bisporus
|
|
5. |
Allium sativum
|
|
6. |
Aloe vera
|
|
7. |
Alpinia officinarum
|
|
8. |
Argyeria speciosa
|
|
9. |
Asparagus adscendens
|
|
10. |
Asparagus racemosus
|
|
11. |
Bacopa monniera
|
|
12. |
Baliospermum montanum
|
|
13. |
Butea monosperma
|
|
14. |
Caesalpinia bonducella
|
|
15. |
Cardiospermum halicacabum
|
|
16. |
Cassia tora
|
|
17. |
Cinnamomum tamala
|
|
18. |
Commiphora mukul
|
|
19. |
Convolvulus pluricaulis
|
|
20. |
Curcuma longa
|
|
21. |
Eclipta prostrata
|
|
22. |
Eugenia jambolena
|
|
23. |
Evolvulus alsinoides
|
|
24. |
Ficus benghalensis
|
|
25. |
Garcinia Cambogia
|
|
26. |
Glycyrrhiza glabra
|
|
27. |
Helicterer irora
|
|
28. |
Hemidesmus indica
|
|
29. |
Indigofera aspalathoides
|
|
30. |
Inula racemosa
|
|
31. |
Leucas cephalotes
|
|
32. |
Madhuca indica
|
|
33. |
Mangifera Indica
|
|
34. |
Momardica charantia
|
|
35. |
Momordica charantia
|
|
36. |
Mucuna pruriens
|
|
37. |
Murraya koenigii
|
|
38. |
Ocimum basilicum
|
|
39. |
Ocimum canum
|
|
40. |
Ocimum gratissimum
|
|
41. |
Ocimum kilimandscharicum
|
|
42. |
Ocimum sanctum
|
|
43. |
Prunus cerasoides
|
|
44. |
Pterocarpus marsupium
|
|
45. |
Rubia cordifolia
|
|
46. |
Semecarpus anacardium
|
|
47. |
Solanum xanthocarpum
|
|
48. |
Symplocos racemosa
|
|
49. |
Terminalia arjuna
|
|
50. |
Terminalia chebula
|
|
51 |
Thuja orientalis
|
|
52. |
Tinospora cordifolia
|
|
53. |
Tridax procumbens
|
|
54. |
Trigonella foenum- graecum
|
|
55. |
Vitex negundo
|
|
56. |
Withania Somnifera
|
|
57. |
Zingiber officinale
|
TOP
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